5' AMP-activated protein kinase (AMPK) is considered metabolic master switch. AMPK is activated by phosphorylation on Thr172 by CaMKK2 and LKB1, among ors. Upon activation, it inhibits synsis of fatty acids, cholesterol, and triglycerides, and activates fatty acid uptake and ß-oxidation as well as stimulating glucose uptake. AMPK is also involved in autophagy activation through direct phosphorylation of ULK1. Revvity's cell-based homogeneous HTRF kit phospho-AMPK (Thr172) enables quantitative detection of AMP-activated protein Kinase modulation, phosphorylated at Threonine 172.